Overview

  • Product nameAnti-C13orf8 antibody
    See all C13orf8 primary antibodies
  • Description
    Rabbit polyclonal to C13orf8
  • SpecificityThis antibody recognizes C13orf8
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: PAASPESRKS ARTTSPEPRK PSPSESPEPW KPFPAVSPEP RRPAPAVSPG, corresponding to internal sequence amino acids 261-310 of Human C13orf8

  • Positive control
    • Daudi cell lysate. Human skeletal muscle cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionRequired for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf8 antibody
    • CAMP antibody
    • CHAMP antibody
    • CHAMP1 antibody
    • CHAP1_HUMAN antibody
    • chromosome 13 open reading frame 8 antibody
    • Chromosome alignment-maintaining phosphoprotein 1 antibody
    • FLJ90413 antibody
    • KIAA1802 antibody
    • Zinc finger protein 828 antibody
    • Zinc finger protein KIAA1802 antibody
    • ZNF828 antibody
    see all

Anti-C13orf8 antibody images

  • Anti-C13orf8 antibody (ab48703) at 0.25 µg/ml + Daudi cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
  • ab48703 (4-8µg/ml) staining of Human muscle tissue cells (10µg) with positively stained skeletal muscle cells (arrows). Magnification: x400

References for Anti-C13orf8 antibody (ab48703)

ab48703 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"