Overview

  • Product name
    Anti-C14orf104 antibody
  • Description
    Rabbit polyclonal to C14orf104
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 (MFSQYAEELT DPENRRRYEA EITALERERG VEVRFVHPEP GHVLRTSLDG) of Human C14orf104 (NP_060609).

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment.
  • Involvement in disease
    Ciliary dyskinesia, primary, 10
  • Sequence similarities
    Belongs to the PIH1 family. Kintoun subfamily.
  • Cellular localization
    Cytoplasm. Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Chromosome 14 open reading frame 104 antibody
    • CILD10 antibody
    • Dnaaf2 antibody
    • Dynein assembly factor 2 antibody
    • FLJ10563 antibody
    • Kintoun antibody
    • KTU_HUMAN antibody
    • Protein kintoun antibody
    see all

Anti-C14orf104 antibody images

  • Species+tissue/cell type:Human nasal epithelial cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa Fluor 546. Secondary antibody dilution:1:1000

  • Anti-C14orf104 antibody (ab99056) at 1 µg/ml + Hela cell lysate at 10 µg

    Predicted band size : 91 kDa
    Gel concentration 6-18%

References for Anti-C14orf104 antibody (ab99056)

This product has been referenced in:
  • Diggle CP  et al. HEATR2 plays a conserved role in assembly of the ciliary motile apparatus. PLoS Genet 10:e1004577 (2014). WB ; Human . Read more (PubMed: 25232951) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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