The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRequired for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment.
Involvement in diseaseCiliary dyskinesia, primary, 10
Sequence similaritiesBelongs to the PIH1 family. Kintoun subfamily.
Cellular localizationCytoplasm. Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia.