Overview

  • Product name
    Anti-C14orf106 antibody
  • Description
    Mouse polyclonal to C14orf106
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human C14orf106 protein (AAH51885.1; or aa 1-314 of Q6P0N0-1 and Q6P0N0-2).

  • Positive control
    • WB: Human spleen tissue lysate, C14orf106 transfected 293T cell lysate ICC/IF: HeLa cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa for isoform 1 and 44 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      C14orf106 is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It interacts with SP1. There are two isoforms.
    • Cellular localization
      Nucleus. Centromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.
    • Database links
    • Alternative names
      • Chromosome 14 open reading frame 106 antibody
      • HsKNL-2 antibody
      • Kinetochore-associated protein KNL-2 homolog antibody
      • M18BP1 antibody
      • Mis18-binding protein 1 antibody
      • MIS18BP1 antibody
      • Putative protein p243 which interacts with transcription factor Sp1 antibody
      • Uncharacterized potential DNA binding protein C14orf106 antibody
      see all

    Images

    • Anti-C14orf106 antibody (ab89265) at 1 µg/ml + human spleen tissue lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 129 , 44 kDa
      Observed band size : ~129 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-C14orf106 antibody (ab89265) at 1 µg/ml

      Lane 1 : C14orf106 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Developed using the ECL technique

      Predicted band size : 129 , 44 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • ab89265, at 10 µg/ml, staining C14orf106 in HeLa cell by Immunofluorescence.

    References

    ab89265 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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