Overview

  • Product nameAnti-C14orf130 antibody
    See all C14orf130 primary antibodies
  • Description
    Rabbit polyclonal to C14orf130
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: MIQCVVCEDW FHGRHLGAIP PESGDFQEMV CQACMKRCSF LWAYAAQLAV, corresponding to internal sequence amino acids 152-201 of Human C14orf130

  • Positive control
    • Jurkat cell lysate and human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceC14orf130 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
  • Database links
  • Alternative names
    • 5730410I19Rik antibody
    • AA589405 antibody
    • AW557761 antibody
    • Chromosome 14 open reading frame 130 antibody
    • EC 6.3.2.- antibody
    • FLJ10483 antibody
    • MGC94098 antibody
    • MGC9518 antibody
    • N recognin 7 antibody
    • Putative E3 ubiquitin-protein ligase UBR7 antibody
    • RGD1359144 antibody
    • Ubiquitin protein ligase E3 component n-recognin 7 (putative) antibody
    • UBR7 antibody
    see all

Anti-C14orf130 antibody images

  • Anti-C14orf130 antibody (ab59151) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling C14orf130 with ab59151 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-C14orf130 antibody (ab59151)

ab59151 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59151.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"