Overview

  • Product name
    Anti-C14orf133 antibody
  • Description
    Rabbit polyclonal to C14orf133
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 443-493 of Human C14orf133 (NP_071350.2).

  • Positive control
    • HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab125084 was affinity purified using an epitope specific to C14orf133 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab125084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Plays a role in lysosomal trafficking. May play a role in epithelial polarization through stabilization of apical membrane protein content, possibly via the RAB11A-dependent apical recycling pathway. Also involved in direct or indirect transcriptional regulation of E-cadherin.
  • Involvement in disease
    Defects in VIPAR are the cause of arthrogryposis-renal dysfunction-cholestasis syndrome type 2 (ARCS2) [MIM:613404]. ARCS2 is an autosomal recessive multisystem disorder, characterized by neurogenic arthrogryposis multiplex congenita, renal tubular dysfunction and neonatal cholestasis with bile duct hypoplasia and low gamma glutamyl transpeptidase activity. Platelet dysfunction is common. Note=In liver, CEACAM5 and ABCB11 are mislocalized and E-cadherin expression is decreased.
  • Sequence similarities
    Belongs to the VIPAR family.
  • Cellular localization
    Early endosome. Recycling endosome. Late endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apical basolateral polarity regulator antibody
    • FLJ12707 antibody
    • hSPE-39 antibody
    • Protein spe-39 homolog antibody
    • SPE 39 antibody
    • SPE 39 protein antibody
    • SPE39 antibody
    • Uncharacterized protein C14orf133 antibody
    • vipar antibody
    • VIPAR_HUMAN antibody
    • VPS16B antibody
    • VPS33B interacting protein antibody
    • VPS33B interacting protein apical basolateral polarity regulator antibody
    • VPS33B interacting protein involved in polarity and apical protein restriction antibody
    • VPS33B-interacting protein antibody
    see all

Images

  • All lanes : Anti-C14orf133 antibody (ab125084) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 57 kDa


    Exposure time : 30 seconds
  • Detection of C14orf133 by Western Blot of Immunprecipitate.
    ab125084 at 1µg/ml staining C14orf133 in HeLa whole cell lysate immunoprecipitated using ab125084 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab125084 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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