Overview

  • Product name
    Anti-C14orf151 antibody
  • Description
    Rabbit polyclonal to C14orf151
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 88-232 of Human C14orf151 (Q27J81).

  • Positive control
    • C14orf151 transfected HEK293T cells, Human stomach tissue, U-251MG cells

Properties

Applications

Our Abpromise guarantee covers the use of ab121114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function
    Severs actin filaments and accelerates their polymerization and depolymerization.
  • Tissue specificity
    Widely expressed. In the kidney, expression is apparent in podocytes and some tubule cells.
  • Involvement in disease
    Defects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5) [MIM:613237]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities
    Belongs to the formin homology family.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
    Contains 1 WH2 domain.
  • Domain
    The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.
    Regulated by autoinhibition due to intramolecular GBD-DAD binding.
    The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf151 antibody
    • C14orf173 antibody
    • CMTDIE antibody
    • DKFZp762A0214 antibody
    • FLJ22056 antibody
    • FSGS5 antibody
    • HBEAG binding protein 2 binding protein C antibody
    • HBEBP2 binding protein C antibody
    • HBEBP2-binding protein C antibody
    • INF 2 antibody
    • inf2 antibody
    • INF2_HUMAN antibody
    • Inverted formin 2 antibody
    • Inverted formin FH2 and WH2 domain containing antibody
    • Inverted formin-2 antibody
    • MGC13251 antibody
    • pp9484 antibody
    see all

Images

  • All lanes : Anti-C14orf151 antibody (ab121114) at 1/250 dilution

    Lane 1 : vector only transfected HEK293T lysate
    Lane 2 : C14orf151 transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

    Developed using the ECL technique
  • ab121114 at 1/60 dilution staining C14orf151 in Paraffin-emebedded Human stomach tissue by Immunohistochemistry. Note: strong cytoplasmic positivity in glandular cells.
  • Immunofluorescent staining ofC14orf151 in U-251MG cells using ab12114 at a concentration of 4 µg/ml. Note: shows positivity in nucleus & cytoplasm.

References

ab121114 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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