Anti-C14orf169 / NO66 antibody (ab113975)


  • Product nameAnti-C14orf169 / NO66 antibody
    See all C14orf169 / NO66 primary antibodies
  • Description
    Rabbit polyclonal to C14orf169 / NO66
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human C14orf169/ NO66 aa 50-100.
    Database link: Q9H6W3

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates



Our Abpromise guarantee covers the use of ab113975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 2-10 µg/mg of lysate.


  • FunctionHistone demethylase that specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the MINA53/NO66 family. NO66 subfamily.
    Contains 1 JmjC domain.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L8 histidine hydroxylase antibody
    • C14orf169 antibody
    • Chromosome 14 open reading frame 169 antibody
    • Histone lysine demethylase NO66 antibody
    • hsNO66 antibody
    • Hypothetical protein FLJ21802 antibody
    • Lysine-specific demethylase NO66 antibody
    • MAPJD antibody
    • MYC associated protein with JmjC domain antibody
    • NO66 antibody
    • NO66_HUMAN antibody
    • Nucleolar protein 66 antibody
    • Ribosomal oxygenase NO66 antibody
    • ROX antibody
    • Up regulated in lung cancer 2 antibody
    • URLC2 antibody
    see all

Anti-C14orf169 / NO66 antibody images

  • All lanes : Anti-C14orf169 / NO66 antibody (ab113975) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 71 kDa

    Exposure time : 30 seconds
  • Detection of C14orf169 / NO66 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113975 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-C14orf169 / NO66 antibody (ab113975)

ab113975 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (hEK293)
Total protein in input 300 µg
Immuno-precipitation step Other - protein G dynabeads
Specification hEK293

Dr. Edyta Marcon

Verified customer

Submitted Jun 26 2015