Overview

  • Product name
    Anti-C14orf169 / NO66 antibody
    See all C14orf169 / NO66 primary antibodies
  • Description
    Mouse polyclonal to C14orf169 / NO66
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Full length protein, corresponding to amino acids 1-641 of Human C14orf169/ NO66 (AAH11350.1).

  • Positive control
    • C14orf169 / NO66 transfected 293T cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Histone demethylase that specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the MINA53/NO66 family. NO66 subfamily.
    Contains 1 JmjC domain.
  • Cellular localization
    Nucleus > nucleolus. Nucleus > nucleoplasm. Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L8 histidine hydroxylase antibody
    • C14orf169 antibody
    • Chromosome 14 open reading frame 169 antibody
    • Histone lysine demethylase NO66 antibody
    • hsNO66 antibody
    • Hypothetical protein FLJ21802 antibody
    • Lysine-specific demethylase NO66 antibody
    • MAPJD antibody
    • MYC associated protein with JmjC domain antibody
    • NO66 antibody
    • NO66_HUMAN antibody
    • Nucleolar protein 66 antibody
    • Ribosomal oxygenase NO66 antibody
    • ROX antibody
    • Up regulated in lung cancer 2 antibody
    • URLC2 antibody
    see all

Images

  • All lanes : Anti-C14orf169 / NO66 antibody (ab169596) at 1 µg/ml

    Lane 1 : C14orf169 / NO66 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 71 kDa
  • Immunofluorescent analysis of HeLa cells labeling C14orf169 / NO66 with ab169596 at 10 µg/ml.

References

ab169596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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