Overview

  • Product nameAnti-C14orf169 / NO66 antibody
    See all C14orf169 / NO66 primary antibodies
  • Description
    Rabbit polyclonal to C14orf169 / NO66
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Human C14orf169/ NO66 (N terminal).
    Sequence:

    DDLGDALPGGAAVAAVPDAARREPYGHLGPAELLEASPAARSLQTPSARL


    Database link: Q9H6W3

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab25941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the MINA53/NO66 family. NO66 subfamily.
    Contains 1 JmjC domain.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L8 histidine hydroxylase antibody
    • C14orf169 antibody
    • Chromosome 14 open reading frame 169 antibody
    • Histone lysine demethylase NO66 antibody
    • hsNO66 antibody
    • Hypothetical protein FLJ21802 antibody
    • Lysine-specific demethylase NO66 antibody
    • MAPJD antibody
    • MYC associated protein with JmjC domain antibody
    • NO66 antibody
    • NO66_HUMAN antibody
    • Nucleolar protein 66 antibody
    • Ribosomal oxygenase NO66 antibody
    • ROX antibody
    • Up regulated in lung cancer 2 antibody
    • URLC2 antibody
    see all

Anti-C14orf169 / NO66 antibody images

  • Anti-C14orf169 / NO66 antibody (ab25941) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

References for Anti-C14orf169 / NO66 antibody (ab25941)

ab25941 has not yet been referenced specifically in any publications.

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