Overview

  • Product nameAnti-C14orf169 / NO66 antibody
    See all C14orf169 / NO66 primary antibodies
  • Description
    Goat polyclonal to C14orf169 / NO66
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide corresponding to Human C14orf169/ NO66 aa 463-473 (internal sequence).
    Sequence:

    C-GAQHSDSKDPR


    Database link: Q9H6W3

  • Positive control
    • A431 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the MINA53/NO66 family. NO66 subfamily.
    Contains 1 JmjC domain.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L8 histidine hydroxylase antibody
    • C14orf169 antibody
    • Chromosome 14 open reading frame 169 antibody
    • Histone lysine demethylase NO66 antibody
    • hsNO66 antibody
    • Hypothetical protein FLJ21802 antibody
    • Lysine-specific demethylase NO66 antibody
    • MAPJD antibody
    • MYC associated protein with JmjC domain antibody
    • NO66 antibody
    • NO66_HUMAN antibody
    • Nucleolar protein 66 antibody
    • Ribosomal oxygenase NO66 antibody
    • ROX antibody
    • Up regulated in lung cancer 2 antibody
    • URLC2 antibody
    see all

Anti-C14orf169 / NO66 antibody images

  • Anti-C14orf169 / NO66 antibody (ab77552) at 0.3 µg/ml + A431 cell lysate in RIPA buffer at 35 µg

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-C14orf169 / NO66 antibody (ab77552)

ab77552 has not yet been referenced specifically in any publications.

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