Overview

  • Product nameAnti-C14orf68 antibody
  • Description
    Goat polyclonal to C14orf68
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-KSRLQADGQGQRR

    , corresponding to internal sequence amino acids 242-254 of Human C14orf68 (NP_997000.2).

  • Positive control
    • Human Liver tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77196 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    IHC-P: Use at a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa.
    Western Blot: Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-C14orf68 antibody images

    • ab77196 at 2.5µg/ml staining C14orf68 in human liver by Immunohistochemistry, paraffin-embedded tissue. Note staining of nucleoli in select hepatocytes.

    References for Anti-C14orf68 antibody (ab77196)

    ab77196 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"