Overview

  • Product nameAnti-C15orf42 antibody
    See all C15orf42 primary antibodies
  • Description
    Rabbit polyclonal to C15orf42
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 975-1025 of Human C15orf42 (NP_689472.3).

  • Positive control
    • 293T whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab124268 was affinity purified using an epitope specific to C15orf42 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab124268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 211 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionRegulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating to CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation.
    • Sequence similaritiesBelongs to the treslin family.
    • Cellular localizationNucleus. Associates with chromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • C15orf42 antibody
      • chromosome 15 open reading frame 42 antibody
      • replication-stimulating protein antibody
      • SLD3 antibody
      • SLD3 homolog antibody
      • SLD3, yeast , homolog of antibody
      • TICRR antibody
      • TICRR_HUMAN antibody
      • TopBP1-interacting antibody
      • TopBP1-interacting checkpoint and replication regulator antibody
      • Treslin antibody
      see all

    Anti-C15orf42 antibody images

    • All lanes : Anti-C15orf42 antibody (ab124268) at 1/1000 dilution

      Lane 1 : 293 whole cell lysate treated with control siLUC RNAi
      Lane 2 : 293 whole cell lysate treated with RNAi targeting C15orf42
      Lane 3 : 293 whole cell lysate treated with RNAi targeting C15orf42

      Lysates/proteins at 50 µg per lane.

      Secondary
      Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 211 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa (possible non-specific binding).

      Exposure time : 2 minutes

      This image is courtesy of an anonymous abreview.

      Blocking: 5% milk

    • All lanes : Anti-C15orf42 antibody (ab124268) at 0.4 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg

      Developed using the ECL technique

      Predicted band size : 211 kDa


      Exposure time : 3 minutes

    References for Anti-C15orf42 antibody (ab124268)

    ab124268 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (7.5%)
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Specification HEK293 cells
    Treatment siRNA for 72h
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted May 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"