Overview

  • Product name
  • Description
    Rabbit polyclonal to C16orf62
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-138 of Human C16orf62.

  • Positive control
    • 293T cell lysate, A431 cell lysate, H1299 cell lysate, HeLaS3 cell lysate, Molt-4 cell lysate, Raji cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 110 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes. Seems not to be required for CCC complex stability (PubMed:25355947).
  • Sequence similarities
    Belongs to the UPF0505 family.
  • Cellular localization
    Membrane. Early endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • C16orf62 antibody
    • Chromosome 16 open reading frame 62 antibody
    • CP062_HUMAN antibody
    • DKFZp313M0539 antibody
    • DKFZp434B0212 antibody
    • Esophageal cancer associated protein antibody
    • Esophageal cancer-associated protein antibody
    • FLJ21040; antibody
    • Hypothetical protein LOC57020 antibody
    • MGC16824 antibody
    • UPF0505 protein C16orf62 antibody
    see all

Images

  • All lanes : Anti-C16orf62 antibody (ab97889) at 1/1000 dilution

    Lane 1 : Molt4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 110 kDa



    7.5% SDS PAGE
  • ab97889, at 1/500 dilution, staining C16orf62 by immunohistochemistry (parafin embedded sections), in Cal27 xenograft.

References

This product has been referenced in:
  • McNally KE  et al. Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. Nat Cell Biol 19:1214-1225 (2017). WB . Read more (PubMed: 28892079) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC-34 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
NSC-34 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

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Verified customer

Submitted Oct 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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