The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
ab121402 at 1/50 dilution staining C16orf89 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry. Note: strong nuclear and cytoplasmic and membranous positivity in respiratory epithelial cells.