The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceThe exact function of C17orf39 remains unknown. It is thought to be a subunit of the multiprotein Mediator complex which is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. This gene is located within the Smith-Magenis syndrome region on chromosome 17.