Overview

  • Product nameAnti-C18orf10 antibody
    See all C18orf10 primary antibodies
  • Description
    Rabbit polyclonal to C18orf10
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide from with internal sequence amino acids 107-156: SMYSLPNAPT LADLEDDTHE ASDDQPEKPH FDSRSVIFEL DSCNGSGKVC, of Human C18orf10 (NP_056291)

  • Positive control
    • 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

Anti-C18orf10 antibody images

  • Anti-C18orf10 antibody (ab83914) at 1 µg/ml (in 5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,83 kDa. We are unsure as to the identity of these extra bands.

References for Anti-C18orf10 antibody (ab83914)

ab83914 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"