Customer reviews and FAQs

Overview

  • Product nameAnti-C18orf8 antibody
    See all C18orf8 primary antibodies
  • Description
    Goat polyclonal to C18orf8
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-DKLNHEYKKYLDAEQ

    , corresponding to internal sequence amino acids 414-428 of Human C18orf8 (NP_037458.3)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human bone marrow, spleen or tonsil lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAmmonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 75 kDa).

Target

Anti-C18orf8 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf8 antibody (ab78596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf8 antibody (ab78596)

    ab87596 (5µg/ml) staining of C18orf8 in paraffin embedded Human Small Intestine following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in the enterocytes.

  • Western blot - C18orf8 antibody (ab78596)
    Western blot - C18orf8 antibody (ab78596)
    Anti-C18orf8 antibody (ab78596) at 1/1 dilution + Human breast tissue lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-C18orf8 antibody (ab78596)

ab78596 has not yet been referenced specifically in any publications.

Publishing research using ab78596? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"