Overview

  • Product name
  • Description
    Goat polyclonal to C18orf8
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-DKLNHEYKKYLDAEQ

    , corresponding to internal sequence amino acids 414-428 of Human C18orf8 (NP_037458.3)

  • Positive control
    • Human bone marrow, spleen or tonsil lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 75 kDa).

Target

Images

  • ab87596 (5µg/ml) staining of C18orf8 in paraffin embedded Human Small Intestine following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in the enterocytes.

  • Anti-C18orf8 antibody (ab78596) at 1/1 dilution + Human breast tissue lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References

ab78596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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