Overview

  • Product name
    Anti-C18orf8 antibody
    See all C18orf8 primary antibodies
  • Description
    Goat polyclonal to C18orf8
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-DKLNHEYKKYLDAEQ

    , corresponding to internal sequence amino acids 414-428 of Human C18orf8 (NP_037458.3)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human bone marrow, spleen or tonsil lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 75 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf8 antibody (ab78596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf8 antibody (ab78596)

    ab87596 (5µg/ml) staining of C18orf8 in paraffin embedded Human Small Intestine following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in the enterocytes.

  • Western blot - C18orf8 antibody (ab78596)
    Western blot - C18orf8 antibody (ab78596)
    Anti-C18orf8 antibody (ab78596) at 1/1 dilution + Human breast tissue lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 75 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

References

ab78596 has not yet been referenced specifically in any publications.

Publishing research using ab78596? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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