Recombinant
RabMAb

Anti-C19orf2 antibody [SP215] - C-terminal (ab183310)

Overview

  • Product name
    Anti-C19orf2 antibody [SP215] - C-terminal
    See all C19orf2 primary antibodies
  • Description
    Rabbit monoclonal [SP215] to C19orf2 - C-terminal
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human C19orf2 (C terminal). The exact sequence is proprietary.
    Database link: O94763

  • Positive control
    • Human 293 cells. Human ovarian adenocarcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Protein A/G purified.
  • Clonality
    Monoclonal
  • Clone number
    SP215
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform Antigen Retrieval by boiling tissue sections in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.
    Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival sigaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity at mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.
  • Tissue specificity
    Ubiquitous. Expressed in ovarian cancers (at protein level). Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells.
  • Sequence similarities
    Belongs to the RNA polymerase II subunit 5-mediating protein family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation occurs essentially on serine residues. Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-372 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-372 disrupts the URI1-PPP1CC complex in the mitochondrion, releaves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity and preventing sustained S6K1-dependent signaling.
  • Cellular localization
    Nucleus. Cytoplasm. Mitochondrion. Cell projection > dendrite. Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 at mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • C19orf2 antibody
    • Chromosome 19 open reading frame 2 antibody
    • NNX3 antibody
    • NNX3 protein antibody
    • PPP1R19 antibody
    • Protein NNX3 antibody
    • Protein phosphatase 1 regulatory subunit 19 antibody
    • RMP antibody
    • RMP_HUMAN antibody
    • RNA polymerase II subunit 5 mediating protein antibody
    • RNA polymerase II subunit 5-mediating protein antibody
    • RPB5 mediating protein antibody
    • RPB5-mediating protein antibody
    • Unconventional prefoldin RPB5 interactor 1 antibody
    • Unconventional prefoldin RPB5 interactor antibody
    • URI antibody
    • URI1 antibody
    • URI1, prefoldin-like chaperone antibody
    see all

Images

  • Immunohistochemical analysis of Human ovarian adenocarcinoma tissue labeling C19orf2 using ab183310 at a 1/100 dilution.

  • Flow cytometric analysis of Human 293 cells labeling C19orf2 using ab183310 at 1/100 (green), compared to a negative control rabbit IgG (blue).

References

ab183310 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183310.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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