• Product nameAnti-C19orf21 antibody
    See all C19orf21 primary antibodies
  • Description
    Rabbit polyclonal to C19orf21
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 578-627 (PTPDENSDQN SRSSSQASGI TGSYSVSESP FFSPIHLHSN VAWTVEDPVD) of Human C19orf21 (NP_775752).

  • Positive control
    • HepG2 cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab81302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionPlays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.
  • Developmental stageRegulated in a cell-cycle dependent manner. Weakly expressed in G1 and S phases. Expression increases in G2/M phases and persisting until the end of mitosis (at protein level).
  • Post-translational
    Phosphorylated by CDK1 and PLK1. CDK1 is the priming kinase for PLK1 phosphorylation. Phosphorylation by PLK1 is required for proper spindle orientation at metaphase.
  • Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Predominantly localizes to cortical actin structures during interphase and mitosis. Present in retraction fibers, which are formed at former adhesion sites during mitosis, and at spicular membrane protrusions in re-attaching cytokinetic cells. Partially colocalizes with cytoplasmic F-actin. Not detected at microtubules at interphase, nor at spindle during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • chromosome 19 open reading frame 21 antibody
    • hypothetical protein LOC126353 antibody
    • Misp antibody
    • MISP_HUMAN antibody
    • Mitotic interactor and substrate of PLK1 antibody
    • Mitotic spindle positioning protein antibody
    • Uncharacterized protein C19orf21 antibody
    see all

Anti-C19orf21 antibody images

  • Anti-C19orf21 antibody (ab81302) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-C19orf21 antibody (ab81302)

ab81302 has not yet been referenced specifically in any publications.

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