Overview

  • Product name
    Anti-C19orf61 antibody
  • Description
    Rabbit polyclonal to C19orf61
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide designed within residues corresponding to amino acids 75 - 125 of Human C19orf61 (NP_061981.2).

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.

Not suitable for crude preparations (e.g. whole cell lysate). Can be used for WB of enriched (e.g. immunoprecipitated) sources of C19orf61.

Target

  • Function
    Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8.
  • Sequence similarities
    Belongs to the SMG9 family.
  • Post-translational
    modifications
    Phosphorylated by SMG1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromosome 19 open reading frame 61 antibody
    • DKFZp564H1322 antibody
    • F17127_1 antibody
    • FLJ12886 antibody
    • Protein smg 9 homolog antibody
    • Protein smg-9 homolog antibody
    • Protein SMG9 antibody
    • SMG 9 antibody
    • smg9 antibody
    • SMG9_HUMAN antibody
    • Suppressor with morphogenetic effect on genitalia protein 9, antibody
    see all

Anti-C19orf61 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling C19orf61 with ab85689 at 1/5000 (0.2µg/ml). Detection: DAB.
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg ab90659.
    20% of the immunoprecipitate was loaded in each lane, and probed with ab90659 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemoluminescence, with an exposure time of 1 second.

References for Anti-C19orf61 antibody (ab85659)

ab85659 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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