Overview

  • Product nameAnti-C1GALT1 antibody
    See all C1GALT1 primary antibodies
  • Description
    Mouse monoclonal to C1GALT1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ETFHPFVPEH HLIKGYLPRT FWYWNYNYYP PVEGPGCCSD LAVSFHYVDS TTMYELEYLV YHLRPYGYLY RYQPTLPERI LKEISQANKN EDTKVKLGNP , corresponding to amino acids 264-364 of Human C1GALT1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceC1GALT1 (Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1) is a glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended O-glycans in glycoproteins. C1GALT1 plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
  • Cellular localizationMembrane; Single-pass type II membrane protein.
  • Database links
  • Alternative names
    • B3Gal T8 antibody
    • Beta 1 3 galactosyltransferase antibody
    • C1GALT antibody
    • Core 1 beta1 3 galactosyltransferase 1 antibody
    • Core 1 beta3 Gal T antibody
    • Core 1 O glycan T synthase antibody
    • Core 1 synthase glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1 3 galactosyltransferase antibody
    • Glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • T synthase antibody
    see all

Anti-C1GALT1 antibody images



  • Predicted band size : 42 kDa
  • Immunofluorescence analysis of Panc1-bC2GnT-M cells, staining C1GALT1 with ab57492.

    Cells were fixed in paraformaldehyde before incubating with primary antibody (1/100) for 1 hour. A DyLight®488-conjugated donkey anti-mouse IgG (1/200) was used as the secondary antibody.

References for Anti-C1GALT1 antibody (ab57492)

This product has been referenced in:
  • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . Read more (PubMed: 25086069) »
  • Petrosyan A  et al. Glycosyltransferase-specific Golgi-targeting Mechanisms. J Biol Chem 287:37621-7 (2012). ICC/IF ; Human . Read more (PubMed: 22988244) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Sample Human Cell lysate - whole cell (LNCaP)
Specification LNCaP
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549)
Specification A549
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Petrosyan

Verified customer

Submitted Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"