Overview

  • Product name
  • Description
    Mouse monoclonal to C1GALT1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ETFHPFVPEH HLIKGYLPRT FWYWNYNYYP PVEGPGCCSD LAVSFHYVDS TTMYELEYLV YHLRPYGYLY RYQPTLPERI LKEISQANKN EDTKVKLGNP , corresponding to amino acids 264-364 of Human C1GALT1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    C1GALT1 (Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1) is a glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended O-glycans in glycoproteins. C1GALT1 plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
  • Cellular localization
    Membrane; Single-pass type II membrane protein.
  • Database links
  • Alternative names
    • B3Gal T8 antibody
    • Beta 1 3 galactosyltransferase antibody
    • C1GALT antibody
    • Core 1 beta1 3 galactosyltransferase 1 antibody
    • Core 1 beta3 Gal T antibody
    • Core 1 O glycan T synthase antibody
    • Core 1 synthase glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1 3 galactosyltransferase antibody
    • Glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • T synthase antibody
    see all

Anti-C1GALT1 antibody images



  • Predicted band size : 42 kDa
  • Immunofluorescence analysis of Panc1-bC2GnT-M cells, staining C1GALT1 with ab57492.

    Cells were fixed in paraformaldehyde before incubating with primary antibody (1/100) for 1 hour. A DyLight®488-conjugated donkey anti-mouse IgG (1/200) was used as the secondary antibody.

References for Anti-C1GALT1 antibody (ab57492)

This product has been referenced in:
  • Ho CW  et al. The cytokine-cosmc signaling axis upregulates the tumor-associated carbohydrate antigen Tn. Oncotarget 7:61930-61944 (2016). WB, IF . Read more (PubMed: 27542280) »
  • Petrosyan A  et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). Read more (PubMed: 25605727) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Sample
Human Cell lysate - whole cell (LNCaP)
Specification
LNCaP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Specification
A549
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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