The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
Use at an assay dependent concentration.
RelevanceC1GALT1 (Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1) is a glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended O-glycans in glycoproteins. C1GALT1 plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
Cellular localizationMembrane; Single-pass type II membrane protein.
Glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
T synthase antibody
Anti-C1GALT1 antibody images
Western blot - Anti-C1GALT1 antibody (ab57492)
Predicted band size : 42 kDa
Immunocytochemistry/ Immunofluorescence - Anti-C1GALT1 antibody (ab57492)Image from Petrosyan A et al., J Biol Chem. 2012 Nov 2;287(45):37621-7. doi: 10.1074/jbc.C112.403006. Epub 2012 Sep 17. Fig 2.; doi: 10.1074/jbc.C112.403006; November 2, 2012 The Journal of Biological Chemistry, 287, 37621-37627.
Immunofluorescence analysis of Panc1-bC2GnT-M cells, staining C1GALT1 with ab57492.
Cells were fixed in paraformaldehyde before incubating with primary antibody (1/100) for 1 hour. A DyLight®488-conjugated donkey anti-mouse IgG (1/200) was used as the secondary antibody.
References for Anti-C1GALT1 antibody (ab57492)
This product has been referenced in:
Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res12:1704-16 (2014).
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