Overview

  • Product nameAnti-C1orf103 antibody
    See all C1orf103 primary antibodies
  • Description
    Rabbit polyclonal to C1orf103
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 684-733 (HSKTRQEKRT EMEYYTHEKQ EKGTLNSNAA YEQSHFFNKN YTEDIFPVTP) of Human C1orf103 (NP_060842).

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC Use at an assay dependent concentration.

Target

Anti-C1orf103 antibody images

  • ab90686 staining C1orf103 in Mouse ES cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-C1orf103 antibody (ab90686) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Gel concentration: 12 %

References for Anti-C1orf103 antibody (ab90686)

ab90686 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (mouse ES)
Specification mouse ES
Permeabilization Yes - 0.5% TritonX100
Fixative Paraformaldehyde
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Submitted Nov 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"