Overview

  • Product nameAnti-C1orf105 antibody
    See all C1orf105 primary antibodies
  • Description
    Rabbit polyclonal to C1orf105
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 93-176 (QPRTMKIPDD PKASFENCMS YRMSLHQPKF QTTPEPFHDD IPTESIHYRL PILGPRTAVF HGLLTEAYKT LKERQRSSLP RKEP) of Human C1orf105.

  • Positive control
    • Human kidney tissue; Transfected HEK293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-C1orf105 antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli. Recommended concentration of ab121993 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C1orf105 antibody (ab121993) at 1/250 dilution

    Lane 1 : Negative control (vector-only transfected HEK293T lysate)
    Lane 2 : C1orf105-transfected HEK293T overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique
  • ab121993, at 1/400, staining C1orf105 in paraffin-embedded Human kidney by Immunohistochemistry.

References for Anti-C1orf105 antibody (ab121993)

ab121993 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"