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Rabbit polyclonal to C1orf135
amino acids 11-93 (CGVWLDAAAL KRRKVQTHLI KPGTKMLTLL PGERKANIYF TQRRAPSTGI HQRSIASFFT LQPGKTNGSD QTSVSSHTES QIN) of Human C1orf135 (Q9H7T9).
- HEK293T transfected lysate.
Human bone marrow tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- C1orf135 antibody
- CA135_HUMAN antibody
- Uncharacterized protein C1orf135 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C1orf135 antibody (ab122014)
Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab122014 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C1orf135 antibody (ab122014)
All lanes : Anti-C1orf135 antibody (ab122014) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : C1orf135 (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf135 antibody (ab122014)
ab122014, at a 1/50 dilution, staining C1orf135 in paraffin-embedded Human bone marrow, by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"