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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-C1orf135 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-C1orf135 antibody (ab122014)
Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab122014 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C1orf135 antibody (ab122014)
All lanes : Anti-C1orf135 antibody (ab122014) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : C1orf135 (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate
Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf135 antibody (ab122014)
ab122014, at a 1/50 dilution, staining C1orf135 in paraffin-embedded Human bone marrow, by Immunohistochemistry.
References for Anti-C1orf135 antibody (ab122014)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"