Overview

  • Product nameAnti-C1orf135 antibody
    See all C1orf135 primary antibodies
  • Description
    Rabbit polyclonal to C1orf135
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 11-93 (CGVWLDAAAL KRRKVQTHLI KPGTKMLTLL PGERKANIYF TQRRAPSTGI HQRSIASFFT LQPGKTNGSD QTSVSSHTES QIN) of Human C1orf135 (Q9H7T9).

  • Positive control
    • HEK293T transfected lysate. Human bone marrow tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-C1orf135 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab122014 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C1orf135 antibody (ab122014) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : C1orf135 (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate

    Developed using the ECL technique
  • ab122014, at a 1/50 dilution, staining C1orf135 in paraffin-embedded Human bone marrow, by Immunohistochemistry.

References for Anti-C1orf135 antibody (ab122014)

ab122014 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"