Overview

  • Product nameAnti-C1orf156 antibody
  • Description
    Rabbit polyclonal to C1orf156
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: NKVIETLPGF QHVKLSVVKT ILLKENFPGE NIVSKSFSSH SDLITGVYEG GLKIWECTFD LLAYFTKAKV KFAGKKVLDL GCGSGL, corresponding to amino acids 115-200 of Human C1orf156.

  • Positive control
    • Human duodenum tissue; C1orf156 HEK293T over-expression lysate
  • General notes


    ab122266 is mono-specific.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesab122266 is mono-specific.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • FunctionProbable histidine methyltransferase.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. METTL18 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arsenic-transactivated protein 2 antibody
    • AsTP2 antibody
    • Histidine protein methyltransferase 1 homolog antibody
    • HPM1 antibody
    • MET18_HUMAN antibody
    • Methyltransferase like 18 antibody
    • Methyltransferase-like protein 18 antibody
    • Mettl18 antibody
    • MGC9084 antibody
    • RP1-117P20.4 antibody
    see all

Anti-C1orf156 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122266 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122266, at 1/10 dilution, staining C1orf156 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-C1orf156 antibody (ab122266) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

References for Anti-C1orf156 antibody (ab122266)

ab122266 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"