The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.
ab119707 at 1/100 dilution staining C1orf187 in Rat PC12 cells, differentiated with NGF, by Immunocytochemistry in the absence (left) or presence (right) of blocking peptide. The lower images show corresponding phase images.
detection used an appropriate secondary antibody conjugated to Cy3.