Recombinant
RabMAb

Anti-C1orf57 antibody [EPR14325] (ab182154)

Overview

  • Product name
    Anti-C1orf57 antibody [EPR14325]
    See all C1orf57 primary antibodies
  • Description
    Rabbit monoclonal [EPR14325] to C1orf57
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human C1orf57 aa 1-150. The exact sequence is proprietary.
    Database link: Q9BSD7

  • Positive control
    • U87-MG and Jurkat cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14325
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/230.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/20.

Target

  • Relevance
    C1orf57 (Chromosome 1 open reading frame 57) belongs to the UPF0334 family. The function of C1orf57 is unknown.
  • Database links
  • Alternative names
    • Chromosome 1 open reading frame 57 antibody
    • FLJ11383 antibody
    • HCR NTPase antibody
    • Human cancer related NTPase antibody
    • Hypothetical protein LOC84284 antibody
    • MGC13186 antibody
    • Novel DUF829 domain containing protein antibody
    • Nucleoside triphosphatase C1orf57 antibody
    • Nucleoside triphosphatase, cancer related antibody
    • Nucleoside triphosphate phosphohydrolase antibody
    • OTTHUMP00000035941 antibody
    • OTTHUMP00000035942 antibody
    • Probable UPF0334 kinase like protein C1orf57 antibody
    • RP4 659I19.2 antibody
    • RP4 678E16.2 antibody
    see all

Images

  • All lanes : Anti-C1orf57 antibody [EPR14325] (ab182154) at 1/2000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa

  • Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling C1orf57 with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab182154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182154.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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