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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
ab117780 was affinity purified using an epitope specific to C1orf80 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
Use at 2-10 µg/mg of lysate.
Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
Belongs to the AIDA family.
Information by UniProt
Anti-C1orf80 antibody images
Western blot - Anti-C1orf80 antibody (ab117780)
All lanes : Anti-C1orf80 antibody (ab117780) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 35 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-C1orf80 antibody (ab117780)
Detection of C1orf80 by Western Blot of Immunprecipitate.
ab117780 at 0.4µg/ml staining C1orf80 in HeLa whole cell lysate immunoprecipitated using ab117780 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-C1orf80 antibody (ab117780)
has not yet been referenced specifically in any publications.
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