Overview

  • Product name
  • Description
    Rabbit polyclonal to C1orf80
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human C1orf80 (NP_073742.2).

  • Positive control
    • Jurkat, HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117780 was affinity purified using an epitope specific to C1orf80 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
  • Tissue specificity
    Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
  • Sequence similarities
    Belongs to the AIDA family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610208M17Rik antibody
    • AIDA antibody
    • AIDA_HUMAN antibody
    • Axin interaction partner and dorsalization antagonist antibody
    • Axin interactor antibody
    • Axin interactor dorsalization associated protein antibody
    • Axin interactor, dorsalization associated antibody
    • Chromosome 1 open reading frame 80 antibody
    • dorsalization-associated protein antibody
    • RP11-378J18.7 antibody
    • UPF0491 protein C1orf80 antibody
    see all

Images

  • All lanes : Anti-C1orf80 antibody (ab117780) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 35 kDa


    Exposure time : 30 seconds
  • Detection of C1orf80 by Western Blot of Immunprecipitate.
    ab117780 at 0.4µg/ml staining C1orf80 in HeLa whole cell lysate immunoprecipitated using ab117780 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab117780 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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