Overview

  • Product nameAnti-C1orf83 antibody
    See all C1orf83 primary antibodies
  • Description
    Mouse polyclonal to C1orf83
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human C1orf83 protein (NP_694580.1).

  • Positive control
    • Jurkat cell lysate. K-562 cell lysate. Cell lysate from transfected 293T cells. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceC1orf83 contains 1 TFIIS central domain and 1 TFIIS N terminal domain. There are two known isoforms.
  • Cellular localizationNucleus
  • Database links
  • Alternative names
    • chromosome 1 open reading frame 83 antibody
    • FLJ32112 antibody
    • FLJ39169 antibody
    • hypothetical protein LOC127428 antibody
    • Uncharacterized protein C1orf83 antibody
    see all

Anti-C1orf83 antibody images

  • Anti-C1orf83 antibody (ab88625) at 1 µg/ml + Jurkat cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Anti-C1orf83 antibody (ab88625) at 1 µg/ml + K-562 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : ~21 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-C1orf83 antibody (ab88625) at 1 µg/ml

    Lane 1 : cell lysate from C1orf83 transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • ab88625 at 10 µg/ml staining C1orf83 in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

References for Anti-C1orf83 antibody (ab88625)

ab88625 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"