Recombinant Anti-C1orf93 antibody [EPR13703] (ab180932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13703] to C1orf93
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C1orf93 antibody [EPR13703]
See all C1orf93 primary antibodies -
Description
Rabbit monoclonal [EPR13703] to C1orf93 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal kidney, HeLa, MCF7 and THP1 cell lines.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13703 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/60.
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WB |
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Notes |
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IP
1/60. |
WB
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Catalyzes the reduction of prostaglandin-ethanolamide H(2) (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha). -
Sequence similarities
Belongs to the prostamide/prostaglandin F synthase family. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 617001 Cow
- Entrez Gene: 127281 Human
- Entrez Gene: 66469 Mouse
- Entrez Gene: 100134955 Pig
- Entrez Gene: 362676 Rat
- SwissProt: Q58CY6 Cow
- SwissProt: Q8TBF2 Human
- SwissProt: Q9DB60 Mouse
see all -
Alternative names
- C1orf93 antibody
- Chromosome 1 open reading frame 93 antibody
- FAM213B antibody
see all
Images
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All lanes : Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/5000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa -
Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/1000 dilution + THP1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa -
Immunoprecipitation of fetal kidney cell line labeling C1orf93 using ab180932 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
All lanes : Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/60 dilution
Lane 1 : fetal kidney cell line at 10 µg
Lane 2 : Negative control
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180932 has not yet been referenced specifically in any publications.