Overview

  • Product name
    Anti-C1q antibody [4.8]
    See all C1q primary antibodies
  • Description
    Rabbit monoclonal [4.8] to C1q
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to Mouse C1q.

  • Positive control
    • IHC: Mouse brain tissue (adult) .
  • General notes

    This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University:

    Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab182451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use a concentration of 1 µg/ml.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease
    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Images

  • Immunohistochemical analysis of perfusion-fixed mouse brain tissue labeling C1q with ab182451 at 1 μg/ml. DAPI staining (blue).

References

This product has been referenced in:
  • Lui H  et al. Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation. Cell 165:921-35 (2016). ICC/IF ; Mouse . Read more (PubMed: 27114033) »
  • Hong S  et al. Complement and microglia mediate early synapse loss in Alzheimer mouse models. Science 352:712-6 (2016). IHC ; Mouse . Read more (PubMed: 27033548) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Post mortem brain tissue)
Permeabilization
Yes - EtOH/H2O2
Specification
Post mortem brain tissue
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 10µg/mL · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 15 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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