• Product nameAnti-C1q antibody [9A7]
    See all C1q primary antibodies
  • Description
    Mouse monoclonal [9A7] to C1q
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    C1q protein purified from Human plasma

  • Positive control
    • K562, Jurkat, C6 and NIH3T3 cell lysates.



Our Abpromise guarantee covers the use of ab71089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


Anti-C1q antibody [9A7] images

  • All lanes : Anti-C1q antibody [9A7] (ab71089) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat T cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab71089 (1µg/ml) staining C1q in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of of the epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-C1q antibody [9A7] (ab71089)

This product has been referenced in:
  • Lui H  et al. Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation. Cell 165:921-35 (2016). Flow Cyt ; Mouse . Read more (PubMed: 27114033) »
  • Astafurov K  et al. Complement expression in the retina is not influenced by short-term pressure elevation. Mol Vis 20:140-52 (2014). WB . Read more (PubMed: 24505213) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Serum (Serum)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Serum
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C

任弘 東岸

Verified customer

Submitted Aug 06 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ventana - cc1mild
Permeabilization No

Abcam user community

Verified customer

Submitted Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Brain)
Loading amount 20 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Thermo SuperBlock Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Dr. Lampros Panagis

Verified customer

Submitted Nov 20 2009