FITC Anti-C1q antibody (ab4223)
Key features and details
- FITC Rabbit polyclonal to C1q
- Suitable for: IHC-P, Flow Cyt, ICC
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-C1q antibody
See all C1q primary antibodies -
Description
FITC Rabbit polyclonal to C1q -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human C1q. C1q isolated from the normal human serum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 21746929
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 21746929 ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. |
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Omim: 120550 Human
- Omim: 120570 Human
- Omim: 120575 Human
- SwissProt: P02745 Human
- SwissProt: P02746 Human
see all -
Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
Images
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C1q isolated from the NHS. This antibody reacts with the Human C1q complement. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.
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ab4223, staining C1q in CHO-K1 cells transfected with M1-AQP4 or M23-AQP4 by Immunocytochemistry/ Immunofluorescence.
Cells were incubated at room temperature with recombinant monoclonal NMO antibody and 120 µg/ml human C1q protein (ab96363). Cells were washed with PBS, fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with primary antibody against C1q (ab4223) at 1/200 dilution.
Datasheets and documents
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Datasheet download
References (8)
ab4223 has been referenced in 8 publications.
- Esaulova E et al. The immune landscape in tuberculosis reveals populations linked to disease and latency. Cell Host Microbe 29:165-178.e8 (2021). PubMed: 33340449
- Pham A et al. Anti-LPS IgA and IgG Can Inhibit Serum Killing of Pseudomonas aeruginosa in Patients with Cystic Fibrosis. Infect Immun 89:e0041221 (2021). PubMed: 34460286
- Aoyama M et al. Effects of terminal galactose residues in mannose a1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies. MAbs 11:826-836 (2019). PubMed: 30990348
- Burbano C et al. Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus. Front Immunol 9:322 (2018). PubMed: 29545790
- Burbano C et al. Extracellular vesicles are associated with the systemic inflammation of patients with seropositive rheumatoid arthritis. Sci Rep 8:17917 (2018). PubMed: 30559453
- Fox S et al. The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection. J Inflamm (Lond) 12:51 (2015). PubMed: 26357509
- Phuan PW et al. Complement-dependent Cytotoxicity in Neuromyelitis Optica Requires Aquaporin-4 Protein Assembly in Orthogonal Arrays. J Biol Chem 287:13829-39 (2012). ICC/IF . PubMed: 22393049
- Barfod L et al. Evasion of immunity to Plasmodium falciparum malaria by IgM masking of protective IgG epitopes in infected erythrocyte surface-exposed PfEMP1. Proc Natl Acad Sci U S A 108:12485-90 (2011). Flow Cyt ; Human . PubMed: 21746929