Overview

  • Product name
    Anti-C1q antibody (FITC)
    See all C1q primary antibodies
  • Description
    Rabbit polyclonal to C1q (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with human C1q complement.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C1q isolated from the normal human serum.

  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab4223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21746929
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22393049

Target

  • Function
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease
    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Images

  • C1q isolated from the NHS. This antibody reacts with the Human C1q complement. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.

  • ab4223, staining C1q in CHO-K1 cells transfected with M1-AQP4 or M23-AQP4 by Immunocytochemistry/ Immunofluorescence.

    Cells were incubated at room temperature with recombinant monoclonal NMO antibody and 120 µg/ml human C1q protein (ab96363). Cells were washed with PBS, fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with primary antibody against C1q (ab4223) at 1/200 dilution.

References

This product has been referenced in:
  • Fox S  et al. The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection. J Inflamm (Lond) 12:51 (2015). Read more (PubMed: 26357509) »
  • Phuan PW  et al. Complement-dependent Cytotoxicity in Neuromyelitis Optica Requires Aquaporin-4 Protein Assembly in Orthogonal Arrays. J Biol Chem 287:13829-39 (2012). ICC/IF . Read more (PubMed: 22393049) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
0.06 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Serum (Serum)
Specification
Serum
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jul 15 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Dog Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Jul 01 2014

Thank you for contacting us.

My colleague has confirmed that we do not currently OEM any of our products for resale. If you are interested in using our products to develop other assays such as kits, my colleagues at mailto:sales@abcam.com can...

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Gracias por contactarnos de nuevo, ¡no es ninguna molestia!

He revisado los artículos referenciados en la datasheet del producto, y para ICC/IF la dilución recomendada es de 1/200. Te copio el párrafo correspondient...

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Approximately 1 mg/ml

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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