Anti-C1QB antibody (ab90181)

Overview

  • Product nameAnti-C1QB antibody
    See all C1QB primary antibodies
  • Description
    Rabbit polyclonal to C1QB
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (GPKGESGDYK ATQKIAFSAT RTINVPLRRD QTIRFDHVIT NMNNNYEPRS) of Human C1QB (NP_000482).

  • Positive control
    • Human fetal stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF 1/600.

Target

Anti-C1QB antibody images

  • ab90181 staining C1QB in Rat brain tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween 29 and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/600 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-C1QB antibody (ab90181) at 1 µg/ml + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa

References for Anti-C1QB antibody (ab90181)

ab90181 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (brain tissue)
Specification brain tissue
Fixative Paraformaldehyde
Permeabilization Yes - TWEEN-20
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C
Username

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Verified customer

Submitted Apr 12 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"