Overview

  • Product name
  • Description
    Rabbit polyclonal to C1r
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human C1r.

    (Peptide available as ab71650.)

  • Positive control
    • This antibody gave a positive signal in Human plasma (total protein)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).

Target

  • Function
    C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Post-translational
    modifications
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1 R antibody
    • C1R antibody
    • C1R_HUMAN antibody
    • Complement C1r antibody
    • Complement C1r subcomponent antibody
    • Complement C1r subcomponent light chain antibody
    • Complement C1r subcomponent precursor antibody
    • Complement component 1 r subcomponent antibody
    • Complement component 1 subcomponent r antibody
    see all

Images

  • All lanes : Anti-C1r antibody (ab66751) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate at 2 µg
    Lane 2 : Human Plasma Total Protein Lysate at 5 µg
    Lane 3 : Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References

This product has been referenced in:
  • Kapferer-Seebacher I  et al. Periodontal Ehlers-Danlos Syndrome Is Caused by Mutations in C1R and C1S, which Encode Subcomponents C1r and C1s of Complement. Am J Hum Genet 99:1005-1014 (2016). Read more (PubMed: 27745832) »
  • Son M  et al. C1q and HMGB1 reciprocally regulate human macrophage polarization. Blood 128:2218-2228 (2016). Read more (PubMed: 27683415) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66751.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up