Overview

  • Product nameAnti-C1r antibody
    See all C1r primary antibodies
  • Description
    Rabbit polyclonal to C1r
  • Specificityab72648 detects a fragment (light chain) of activated C1r resulting from cleavage adjacent to Ile464.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human C1r.

  • Positive control
    • Extracts from 293 cells treated with etoposide (25uM, 1hour) and A549 cells treated with etoposide (25uM, 24hours).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.

Target

  • FunctionC1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Post-translational
    modifications
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1 R antibody
    • C1R antibody
    • C1R_HUMAN antibody
    • Complement C1r antibody
    • Complement C1r subcomponent antibody
    • Complement C1r subcomponent light chain antibody
    • Complement C1r subcomponent precursor antibody
    • Complement component 1 r subcomponent antibody
    • Complement component 1 subcomponent r antibody
    see all

Anti-C1r antibody images

  • All lanes : Anti-C1r antibody (ab72648) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from A549 cells treated with etoposide (25uM, 24hours)
    Lane 3 : Extracts from 293 cells treated with etoposide (25uM, 1hour) with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 19 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-C1r antibody (ab72648)

ab72648 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"