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antigen sequence: LKAREAEAWE EPTELLGLPS ALAGMQDLRL EGALHLLLAQ QQLRARARAG SVRPRGQPTP GEMLPSLPVG SDSEGPENEG TRAALAAAGL SGGRH, corresponding to amino acids 322-416 of Human C20orf151.
Our Abpromise guarantee covers the use of ab122804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labelling C20orf151 with ab122084 at 1-4µg/ml. Shows positivity in nucleus but not nucleoli and vesicles. Cells treated with PFA/Triton X-100.
ab122804 has not yet been referenced specifically in any publications.