Overview

  • Product name
    Anti-C20orf19 antibody
  • Description
    Rabbit polyclonal to C20orf19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 187-438 of C20orf19 (Q2M2Z5)

  • Positive control
    • HCT116 lysate, Human lung papillory adenocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.
  • Sequence similarities
    Belongs to the kizuna family.
  • Post-translational
    modifications
    Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centrosomal protein kizuna antibody
    • DKFZP586H021 antibody
    • HT013 antibody
    • Kiz antibody
    • KIZ_HUMAN antibody
    • KIZUNA antibody
    • MGC102941 antibody
    • MGC141930 antibody
    • NCRNA00153 antibody
    • Plk1s1 antibody
    • Polo-like kinase 1 substrate 1 antibody
    • Uncharacterized protein C20orf19 antibody
    see all

Images

  • Anti-C20orf19 antibody (ab154723) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 75 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human Lung papillory adenocarcinoma tissue labeling C20orf19 with ab154723 at a 1/500 dilution.

References

ab154723 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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