Overview

  • Product name
  • Description
    Rabbit polyclonal to C20orf77
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 75-315 of Human C20orf77 (UniProt ID: Q9NQG5).

  • Positive control
    • 293T, HeLa and HepG2 whole cell lysates; SkHep1 and C2C12 xenograft tissues.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Relevance
    C20orf77 interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It is a transcriptional regulator which enhances expression of CCND1. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It also revents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • C20orf77 antibody
    • Cell cycle related and expression elevated protein in tumor antibody
    • Chromosome 20 open reading frame 77 antibody
    • CREPT antibody
    • dJ1057B20.2 antibody
    • DKFZp434P0735 antibody
    • FLJ44520 antibody
    • Hypothetical protein LOC58490 antibody
    • NET60 antibody
    • Regulation of nuclear pre mRNA domain containing 1B antibody
    • Regulation of nuclear pre mRNA domain containing protein 1B antibody
    • Uncharacterized protein C20orf77 antibody
    see all

Images

  • All lanes : Anti-C20orf77 antibody (ab154910) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SkHep1 xenograft tissue labeling C20orf77 with ab154910 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft tissue labeling C20orf77 with ab154910 at 1/500 dilution.

References

ab154910 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154910.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up