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Rabbit polyclonal to C21ORF62
Predicted to work with:
Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (STNTLPTEYL AICGLKRLRI NMEAKHPFPE QSLLIHSGGD SDSREKPMWL) of Human C21ORF62 (NP_062542).
293T cell lysate.
DU145 cell line (IF/ICC)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The exact function of C21orf62 remains unknown.
Chromosome 21 open reading frame 62 antibody
Western blot - C21ORF62 antibody (ab84746)
Anti-C21ORF62 antibody (ab84746) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
33 kDa (
why is the actual band size different from the predicted?
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence - Anti-C21ORF62 antibody (ab84746)
ICC/IF image of ab84746 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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