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Synthetic peptide: QRKKELIAKL DQAEKEKVDA AELVREFEAL TEENRTLRLA QSQCVEQLEK, corresponding to amino acids 181-230 of Human C21orf7
Our Abpromise guarantee covers the use of ab58637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Titre using peptide based assay: 1/312500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling C21orf7 with ab58637 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.
ab58637 has not yet been referenced specifically in any publications.