Overview

  • Product name
  • Description
    Rabbit polyclonal to C22orf25
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 2-51 (CIIFFKFDPR PVSKNAYRLI LAANRDEFYS RPSKLADFWG NNNEILSGLD) of Human C22orf25 (NP_690870).

  • Positive control
    • Human fetal muscle lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-C22orf25 antibody images

  • Anti-C22orf25 antibody (ab87576) at 1 µg/ml + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa

References for Anti-C22orf25 antibody (ab87576)

ab87576 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (muscle tissue)
Permeabilization
Yes - BSA5%- tween- triton1%
Specification
muscle tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Jun 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (THYROID GLAND)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CITRATE PH6
Permeabilization
No
Specification
THYROID GLAND
Blocking step
Novolink polymer detection system as blocking agent for 30 minute(s) · Concentration: 0.4% · Temperature: 25°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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