The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Use a concentration of 5 - 10 µg/ml.
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Belongs to the RtcB family.
Nucleus. Cytoplasm. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low.
Staining of Human Testis by ab118290 (5μg/mL) in paraffin embedded tissue following stemaed antigen retieval with citrat buffer pH6, AP-Staining this shows nuclear and vicinity staining in spermatogonia.
Western blot - Anti-C22orf28 antibody (ab118290)
Anti-C22orf28 antibody (ab118290) at 0.3 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg Developed using the ECL technique
Predicted band size : 55 kDa Observed band size : 55 kDa