Overview

  • Product nameAnti-C22orf28 antibody
    See all C22orf28 primary antibodies
  • Description
    Goat polyclonal to C22orf28
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    QADPNKVSARAKKR

    with a Cysteine residue linker, corresponding to internal sequence amino acids 201-214 of Human C22orf28 (NP_055121.1)

  • Positive control
    • HeLa, Jurkat and K562 lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab118290 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab118290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionCatalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
  • Sequence similaritiesBelongs to the RtcB family.
  • Cellular localizationNucleus. Cytoplasm. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ankyrin repeat domain 54 antibody
    • Chromosome 22 open reading frame 28 antibody
    • DJ149A16.6 antibody
    • HSPC117 antibody
    • Hypothetical protein LOC51493 antibody
    • Novel protein HSPC117 antibody
    • OTTHUMP00000028639 antibody
    • RP1-149A16.6 antibody
    • rtcB antibody
    • RTCB_HUMAN antibody
    • tRNA-splicing ligase RtcB homolog antibody
    see all

Anti-C22orf28 antibody images

  • Staining of Human Testis by ab118290 (5μg/mL) in paraffin embedded tissue following stemaed antigen retieval with citrat buffer pH6, AP-Staining this shows nuclear and vicinity staining in spermatogonia.

  • Anti-C22orf28 antibody (ab118290) at 0.3 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

References for Anti-C22orf28 antibody (ab118290)

ab118290 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 40 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"