Anti-C22orf28 antibody (ab98231)

Overview

  • Product name
  • Description
    Rabbit polyclonal to C22orf28
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 107-156 (PEAVVSPGGV GFDINCGVRL LRTNLDESDV QPVKEQLAQA MFDHIPVGVG) of Human C22orf28 (NP_055121).

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Anti-C22orf28 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling C22orf28 with ab98231 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - C22orf28. Right - Merge.


  • Predicted band size : 55 kDa
    Western blot analysis of Jurkat cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.


  • Predicted band size : 55 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling C22orf28 with ab98231 at 1.0µg/ml.


  • Predicted band size : 55 kDa
    Western blot analysis of HeLa cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.
  • Anti-C22orf28 antibody (ab98231) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 55 kDa

References for Anti-C22orf28 antibody (ab98231)

ab98231 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
40 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Thank you for your answer and sending the bovine sequence. Indeed, there is a 100% alingment with the immunogen and the chances are therefore very high that the antibody will crossreact. I am thus sending you here the testing discount code. Please see ...

Read More

Thank you very much for your interest in ab98231.

Unfortunately we do not have any trial vials of our products. However if you would like to test this antibody, we can make you an interesting testing offer - please see below.

Do ha...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up