Overview

  • Product name
  • Description
    Rabbit polyclonal to C22orf29
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 71-195 of Human C22orf29 (Q7L3V2).

  • Positive control
    • RT4 cell lysate, Human heart muscle tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Anti-C22orf29 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121113 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C22orf29 antibody (ab121113) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121113 at 1/150 dilution staining C22orf29 in Paraffin-emebedded Human heart muscle tissue by Immunohistochemistry. Note: cytoplasmic positivity in myocytes.

References for Anti-C22orf29 antibody (ab121113)

ab121113 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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