Overview

  • Product nameAnti-C22orf32 antibody
    See all C22orf32 primary antibodies
  • Description
    Rabbit polyclonal to C22orf32
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RWLVLAPVRS GALRSGPSLR KDGDVSAAWS GSGRSLVPSR SVIVTR, corresponding to amino acids 7-52 of Human C22orf32.

  • Positive control
    • Human kidney tissue; HEK293T cell C22orf32 over-expression lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-C22orf32 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab122209 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122209 at 1/150 dilution staining C22orf32 in paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • All lanes : Anti-C22orf32 antibody (ab122209) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : C22orf32 over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

References for Anti-C22orf32 antibody (ab122209)

ab122209 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse heart)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Loading amount 5 µg
Specification Mouse heart
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 4% · Temperature: RT°C
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Verified customer

Submitted Aug 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"