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Product name Anti-C2b antibody
Rabbit polyclonal to C2b
Tested applications Suitable for:
WB more details
Synthetic peptide corresponding to Mouse C2b aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P21180 (Peptide available as ab95402)
This antibody gave a positive signal in Mouse liver tissue lysate.
This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Primary antibody notes This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).
Relevance Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
Cellular localization Secreted
Anti-C2b antibody images
Western blot - C2b antibody (ab66999)
Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 84 kDa Observed band size : 97 kDa ( why is the actual band size different from the predicted?) Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
References for Anti-C2b antibody (ab66999)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"