Overview

  • Product nameAnti-C2b antibody
  • Description
    Rabbit polyclonal to C2b
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse C2b aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P21180
    (Peptide available as ab95402)

  • Positive control
    • This antibody gave a positive signal in Mouse liver tissue lysate.
  • General notes


    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThis antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).

Target

  • RelevanceComponent C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • C2 antibody
    • C2b antibody
    • CO2 antibody
    • complement C2 antibody
    • Complement C2b fragment antibody
    • complement component C2 antibody
    see all

Anti-C2b antibody images

  • Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-C2b antibody (ab66999)

ab66999 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66999.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"