Overview

  • Product name
    Anti-C2b antibody
  • Description
    Rabbit polyclonal to C2b
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse C2b aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P21180
    (Peptide available as ab95402)

  • Positive control
    • This antibody gave a positive signal in Mouse liver tissue lysate.
  • General notes


    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).

Target

  • Relevance
    Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • C2 antibody
    • C2b antibody
    • CO2 antibody
    • complement C2 antibody
    • Complement C2b fragment antibody
    • complement component C2 antibody
    see all

Images

  • Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab66999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66999.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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