C2C12 nuclear extract lysate (ab14639)

Overview

  • Product name
    C2C12 nuclear extract lysate
    See all C2C12 lysates
  • General notes
    Cell line: C2C12 (Mouse myoblast).
    We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).

References

ab14639 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. The C2C12 cells are seeded as undifferentiated. They differentiate by themselves when they come at confluence (confluency induces differentiation). Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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