Overview

  • Product nameAnti-C2ORF25 antibody
    See all C2ORF25 primary antibodies
  • Description
    Rabbit polyclonal to C2ORF25
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 215-264 (RAEGYWADFI DPSSGLAFFG PYTNNTLFET DERYRHLGFS VDDLGCCKVI) of Human C2ORF25 (NP_056517)

  • Positive control
    • human Fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 39, 50 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in cobalamin metabolism.
  • Tissue specificityWidely expressed at high levels.
  • Involvement in diseaseDefects in MMADHC are the cause of methylmalonic aciduria and homocystinuria type cblD (MMAHCD) [MIM:277410]. A disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Clinical features include developmental delay, hyotonia, mental retardation, seizures, megaloblastic anemia. Some patients manifest combined methylmalonic aciduria and homocystinuria (referred to as cblD original), some have only isolated homocystinuria (cblD variant 1), and others have only methylmalonic aciduria (cblD variant 2).
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2orf25 antibody
    • cblD antibody
    • Chromosome 2 open reading frame 25 antibody
    • CL25022 antibody
    • Methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria antibody
    • Methylmalonic aciduria and homocystinuria type D protein antibody
    • methylmalonic aciduria and homocystinuria type D protein, mitochondrial antibody
    • mitochondrial antibody
    • MMAD_HUMAN antibody
    • Mmadhc antibody
    • Protein C2orf25, mitochondrial antibody
    see all

Anti-C2ORF25 antibody images

  • Anti-C2ORF25 antibody (ab83554) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 39,50 kDa (why is the actual band size different from the predicted?)

References for Anti-C2ORF25 antibody (ab83554)

ab83554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"